Cat#: 123P40
序列（三字母）：(H-Asp-Glu-Val-Asp)₂-Rhodamine 110 trifluoroacetate salt
序列（单字母）：(DEVD)₂-R110
类似名：(DEVD)₂-R110, (Asp-Glu-Val-Asp)2-Rhodamine 110
分子式：C₅₆H₆₆N₁₀O₂₃
分子量：1247.19
CAS#：220846-75-3
合成方法：Synthetic
存储条件：-20 ± 5 °C
应用：Apoptosis & Necrosis
描述：(DEVD)₂-R110, non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide, is a highly sensitive, photostable fluorogenic substrate for the determination of caspase-3 activity. Characteristic for substrates based on Rhodamine 110 (R110) is that they contain two identical amino acids or peptides attached to a single fluorophor thereby suppressing its visible absorption and its fluorescence. Upon enzymatic cleavage, this substrate is converted first to the fluorescent monoamide and then to the even more fluorescent R110. The spectral properties of these hydrolysis products are similar to those of fluorescein, with peak excitation and emission wavelengths of 496 and 520 nm, respectively. The fluorescence intensity can be measured by flow cytometry, microwell plate reader, or fluorescence microscopy. Useful for high throughput screening applications.