Cat#：140P55
序列（三字母）：AMCA-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH₂
序列（单字母）：AMCA-EEKPISFFRLGK(biotinyl)-NH₂
类似名：(7-Amino-4-methylcoumarin-3-yl)acetyl-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH₂
分子式：C₉₀H₁₃₁N₂₁O₂₂S
分子量：1891.22
CAS#：851231-19-1
合成方法：Synthetic
存储条件：-20 ± 5 °C
应用：Biotinylated Peptides
描述：Specific substrate for cathepsin D (Km = 53 µM, kcat = 7.28 s⁻¹, kcat/Km = 0.14 µM⁻¹s⁻¹) with a single cathepsin cleavage site between the two Phe residues. It is flanked by an N-terminal AMCA-fluorophore (7-amino-4-methylcoumarin-3-acetic acid) and a C-terminal biotin moiety. After enzymatic hydrolysis the biotin-conjugated cleavage product and undigested substrate AMCA-EEKPISFFRLGK(Biotinyl)-amide can be removed with streptavidin-coated magnetic beads. The remaining fluorescent peptide in solution represents the amount of digested substrate. The versatility of this cathepsin D ´digest and pull down assay´ is applicable for routine assays.