Cat#：173P11
序列（三字母）：Dnp-Pro-β-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(N-Me-Abz)-NH₂
序列（单字母）：Dnp-P-β-cyclohexyl-AGC(Me)HAK(N-Me-Abz)-NH₂
类似名：MMP-1/MMP-9 Substrate, Fluorogenic
分子式：C₄₉H₆₈N₁₄O₁₂S
分子量：1077.23
CAS#：150956-92-6
合成方法：Synthetic
存储条件：-20 ± 5 °C
应用：Cancer Research| Inflammation Research
描述：Efficient fluorogenic substrate for two matrix metalloproteinases: interstitial collagenase (MMP-1) and gelatinase (MMP-9). Dnp-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys(N-Me-Abz)-amide has favorable solubility characteristics. Both enzymes cleave this substrate between Gly and Cys(Me) (Smc), liberating a cleavage product with a fluorescence signal suitable for inhibitor screening and determining Ki values. The major advantage of this FRET substrate is its adaptability to filters commonly available on commercial plate readers (excitation at 365 nm and emission at 450 nm).