Cat#：173P13
序列（三字母）：Dnp-Pro-Leu-Gly-Cys(Me)-His-Ala-D-Arg-NH₂
序列（单字母）：Dnp-PLGC(Me)HAr-NH₂
类似名：MMP-1 Substrate II
分子式：C₃₈H₅₇N₁₅O₁₁S
分子量：932.03
CAS#：145224-98-2
合成方法：Synthetic
存储条件：-20 ± 5 °C
应用：Cancer Research| Inflammation Research
描述：In an approach to optimize substrate specificity, two amino acids have been substituted at the cleavage site of the collagenase substrate, Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH₂. The substrate possesses a 10-fold better kcat/Km value for human fibroblast collagenase than the FRET substrate Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH₂. However, monitoring of the enzyme activity using Dnp-Pro-Leu-Gly-Cys(Me)-His-Ala-D-Arg-NH₂ requires HPLC analysis as the quencher (Trp) has been removed by the amino acid substitutions.