Cat#：173P21
序列（三字母）：Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH₂ trifluoroacetate salt
序列（单字母）：Mca-PLGL-Dpa-AR-NH₂
类似名：7-Methoxycoumarin-4-Acetyl-Pro-Leu-Gly-Leu-Dnp-(2,3-Diaminopropionyl)-Ala-Arg, amide
分子式：C₄₉H₆₈N₁₄O₁₅
分子量：1093.16
CAS#：140430-53-1
合成方法：Synthetic
存储条件：-20 ± 5 °C
应用：Cancer Research
描述：Mca-PLGL-Dpa-AR-amide is a very sensitive FRET substrate for continuous assays and in situ determination of the matrix metalloproteinase activity. Cleavage at the Gly-Leu bond separates the highly fluorescent Mca group from the efficient 2,4-dinitrophenyl quencher, resulting in an increase in fluorescence intensity. The kcat/Km values for the punctuated metalloproteinase (MMP-7) and for gelatinase (MMP-2), e.g., are 1.7·10⁵ and 6.3·10⁵ M⁻¹s⁻¹, respectively.